Project: Gorgon !!HOT!! Download 12 Mb 💪

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Project: Gorgon Download 12 Mb

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Contact Us: Global Environmental Energy Research Institute (GEERI).Q:

What can cause the membrane potential in voltage clamp experiments to drop to zero?

Let’s say we have the membrane potential of a neuron down to a value of -100 mV. If we clamped our membrane potential at this value using the voltage clamp and using the whole-cell patch, what can cause this membrane potential to drop to zero in a jiffy?


The cell is poisoned and has no current. The voltage clamp amplifier becomes unstable.
Addressing this issue requires the patch-clamp technique to be stable for minutes, if not longer.
The patch-clamp amplifier used in your description can take at least a second to reset (sometimes longer) and that’s a lot longer than desired, hence the clamps are unstable.


To answer the question as per edited wording:

If we clamped our membrane potential at this value using the voltage clamp and using the whole-cell patch, what can cause this membrane potential to drop to zero in a jiffy?

if the recorded membrane potential reaches zero what you have recorded is not a ‘biological’ potential but rather an artifact.
If you use a loose voltage clamp and sweep the voltage from -100mV to +100mV you’ll see voltage jumps, hundreds of pA, that occur synchronous with the opening and closing of the voltage clamp.
You can see this nicely in the figure below where the potential sweeps through the membrane and when it reaches -100m

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