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In SharePoint 2010, web parts can either be customized using JavaScript, JQuery or VBscript. For more information on JavaScript and JQuery web parts, read up on SharePoint JavaScript, JQuery and AJAX Web Parts. You can read about SharePoint web part customization using VBscript by reading about Customizing a SharePoint 2007 web part.Silencing the P2X7 receptor inhibits M2 macrophage polarization and pulmonary injury in animal models of COPD.
Macrophages, a heterogeneous population of immune cells, are important components of the lung immune system. In this study, we aimed to explore the role of the P2X7 receptor in macrophages and lung injury in COPD. Phosphorylated P2X7 receptor was evaluated by immunohistochemistry staining in the lung tissue and the localization of P2X7 receptor in macrophages was confirmed by immunofluorescence staining. Then, we knocked down the P2X7 receptor in RAW264.7 cells by using small interference RNA and evaluated the effects of P2X7 receptor silencing on the polarization of macrophages. The mice model of chronic obstructive pulmonary disease (COPD) was established by cigarette smoke and lipopolysaccharide treatment. The forced expiratory volume in 1 second (FEV1) was determined by forced oscillation technique, and the pulmonary pathology was evaluated by hematoxylin-eosin (HE) staining. The expressions of M1 markers such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, and M2 markers such as IL-4, IL-13 and CD206 in the macrophages were detected by quantitative polymerase chain reaction and flow cytometry. In RAW264.7 cells, P2X7 receptor was mainly expressed on the cell membrane, and its overexpression promoted macrophage polarization. In the animal model of COPD, the overexpression of the P2X7 receptor was enhanced in the airway epithelial cells and macrophages, and the severity of pulmonary injury and 4f8c9c8613

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